Ramavath Mohanbabu Naik

ABSTRACTLinum Usitatissimum (LU) has a lipid-lowering action in both normal and diabetic animals. Because OS leaves are rich in oil, the present study was conducted to explain the anti-hyperlipidemic and organ-protective effect of LU fixed oil in rats fed with a high fat (HF) diet. LU fixed oil was extracted by hexane and the fatty acids composition identified by GC-MS. Four groups of male Wistar rats included a normal control group, a high fat fed-diet (HF) group, a HF group treated with LU fixed oil, and a HF group treated with a reference drug Atorvastatin. The results show that LU fixed oil contains five kinds of fatty acids, of which alpha-linolenic acid was the major fatty acid. LU fixed oil depressed high serum levels of total cholesterol, triglyceride, LDL-C, and AI, whereas no significant effect on HDL-C was observed. LU fixed oil also suppressed high levels of liver cholesterol and triglyceride with no significant effect on both lipids in feces. In addition, LU fixed oil normalized the high serum levels of LDH and CK-MB but no significant effect on high serum levels of ALT, AST, and ALP was obtained. We conclude that treatment with LU fixed oil during the last three weeks of HF diet feeding decreased the high serum lipid profile and expressed antiartherogenic and cardioprotective actions against hyperlipidemia. The anti-hyperlipidemic action of OS fixed oil was mainly resulted from the suppression of liver lipid synthesis. Linolenic acid and linoleic acid contained in LU fixed oil were possibly responsible for both lipid-lowering and cardiac protective action against hyperlipidemia. Keywords: Anti-Hyperlipidemic Activity, Linum Usitatissimum, Linolenic Acid, Atorvastatin.

ABSTRACTThe Chromatographic condition were successfully developed for the separation of Acyclovir and     Hydrocortisone by using Agilent C8 column (25 cm x 4.6 mm i.d., 5 ?). A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Hydrocortisone and Acyclovir from pharmaceutical formulation. The method was carried out on a C8 (25 cm x 4.6 mm i.d., 5 ?) column with a mobile phase consisting of Methanol: water (adjusted to pH 3.0 using o-phosphoric acid) in the ratio of 80:20 v/v. The retention time of  Hydricortusone and Acyclovir was 3.50 min and 6.00 min respectively with the flow rate of 1mL/ min. Eluents were detected at 254 nm.  The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for Hydrocortisone and Acyclovir were R2=0.9995 and R2=0.9996 in the concentration range of 10-40µg. mL-1 , 20-80 µg. mL-1 respectively. The relative standard deviation for intra-day precision was lower than 2.0 %. The method was validated according to the ICH guidelines. The method was also found to be robust. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. Keywords: RP-HPLC, Hydrocortisone, Acyclovir, Agilent , Mobile Phase, Retention time.