Hydrocortisone

ABSTRACTThe Chromatographic condition were successfully developed for the separation of Acyclovir and     Hydrocortisone by using Agilent C8 column (25 cm x 4.6 mm i.d., 5 ?). A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Hydrocortisone and Acyclovir from pharmaceutical formulation. The method was carried out on a C8 (25 cm x 4.6 mm i.d., 5 ?) column with a mobile phase consisting of Methanol: water (adjusted to pH 3.0 using o-phosphoric acid) in the ratio of 80:20 v/v. The retention time of  Hydricortusone and Acyclovir was 3.50 min and 6.00 min respectively with the flow rate of 1mL/ min. Eluents were detected at 254 nm.  The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for Hydrocortisone and Acyclovir were R2=0.9995 and R2=0.9996 in the concentration range of 10-40µg. mL-1 , 20-80 µg. mL-1 respectively. The relative standard deviation for intra-day precision was lower than 2.0 %. The method was validated according to the ICH guidelines. The method was also found to be robust. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. Keywords: RP-HPLC, Hydrocortisone, Acyclovir, Agilent , Mobile Phase, Retention time.

Atopic dermatitis (AD) is a common skin disease in children, a chronic, pruritic inflammatory skin disease that usually starts in early infancy. But also affects many adults. It follows a relapsing course, that adversely affects the quality of life of patients and their families. This study was carried out to evaluate and compare the efficacy of hydrocortisone alone and its combination therapy with rainwater for the treatment of atopic dermatitis. This prospective, comparable study was carried out in the dermatological department of Baqubah teaching hospital, outpatient clinic. A total of 120 patients of (1-12 years) old attending outpatient clinic and diagnosed by dermatological specialist as cases of moderate to severe atopic dermatitis from October 2016 to January 2107 were selected and randomized into two groups of 60 patients of each group, Group -A treated with combination therapy of hydrocortisone with rainwater and Group-B  treated with hydrocortisone alone for 8 weeks. Patients were assessed for severity according to the SCORing Atopic Dermatitis (SCORAD) Index. Subsequent examination at weekly interval was used to evaluate the efficacy of the treatment for 8 weeks. A p-value lower than 0.05 was considered statistically significant. A total of 117 patients were completed the study period of 8 weeks (group –A,59 and Group-B,58). A statistically significant difference was observed in the family history of atopic dermatitis among both groups. Significant response was observed at the end of 8 weeks of treatment, in Group-A (96.61%), while in Group-B (87.93%), according to the clinical assessment grades of atopic dermatitis , and during  the follow up, a statistically significant decrease in SCORing Atopic Dermatitis (SCORAD) in comparison of the two groups. Group –A was found to respond significantly better in term of mean percentage decrease in SCORAD as compared with Group-B during the whole study period (P value