S. Ramanaiah1 and P. Venkateswarlu

ABSTRACTA simple, sensitive and rapid spectrophotometric method has been developed for the estimation of Amphetamine (AM) in bulk and in pharmaceutical formulations using 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ). The proposed method is based on the formation of charge-transfer complex between AM and DDQ. AM in the presence of DDQ formed pale wine red color complex which showed a maximum absorbance at 465 nm against the blank. The limit of detection and quantitation were 0.1395 mg/ml and 0.4644 mg/ml respectively. The results of analysis were validated by recovery studies, accuracy, precision, LOD, LOQ, robustness and ruggedness, which indicated that the present method can be successfully applied for the determination of AM in pure and in pharmaceutical formulations. Keywords: Spectrophotometric method, Amphetamine, Charge-transfer complex, DDQ.