HPLC.

The genus Salvia, composed of more than 900 species worldwide distributed, is well known for its various uses, including therapeutic ones. Many species within the genus exhibit activities such as antioxidant, anti-inflammatory, antimicrobial etc. The chemical composition of these species includes polyphenols, flavonoids, terpenes, which induce such activities. The present study compares the polyphenolcarboxilic acids and flavonoids content of 3 species of Salvia present in Ukrainian flora, including S. patens L. and S. verticillata L. Using a HPLC method, polyphenolcarboxilic acids and flavonoid compounds were determined in S. officinalis L., S. patens L. and S. verticillata L.

In this study, work was done for the development of new precise, accurate and sensitive analytical methods for the evaluation of Meloxicam by reverse and normal phase HPLC. After development of two separate methods they are compared to determine the more authentic method of the two. In reverse phase HPLC, C18 column was used and mobile phase was acetonitrile and methanol. Similarly chloroform was used as mobile phase with silicon column for normal phase HPLC. Separately equal volume of standard solution and sample solutions in HPLC vials were injected in auto sampler compartment of HPLC. Chromatogram peak areas of Meloxicam in standard and sample solutions of different concentrations were recorded and compared. These methods are later validated in different ways. The calibration curve proved to have linearity coefficient of 0.999 for reverse and 0.994 for normal phase HPLC. The precision was equivalent to 0.0003%for reverse and 0.003%for normal phase. The LOD and LOQ were 0.0003ug/ml and 0.001ug/ml respectively for reverse and 0.002 and 0.006ug/ml for normal phase. The system also showed accuracy over the range of 95 to 99% for reverse and 91 to 97% for normal phase. These methods showed accuracy, reproducibility and sensitivity and reverse phase proved to be better than normal phase but both can be used alternatively. Key words: Meloxicam, acetonitrile, methanol, HPLC.