Linearity

ABSTRACTThe aim of the present work was to develop and validate a simple and efficient method for the analysis of Aprepitant in pharmaceutical dosage forms by reverse phase high-pressure liquid chromatography. A stainless steel column 75 mm long, 4.6 mm internal diameter filled with octasilyl silica chemically bonded with synthetic hybrid silica gel particles of 3.5 mm diameter was used for elution. The retention time of Aprepitant was 4.05 min. The method showed a good linearity in the concentration range of 0.02478 – 0.07434 mg/mL with a correlation coefficient of 0.9999. The validation characteristics included specificity, linearity, limit of detection, limit of quantification, precision, robustness and stability. Validation acceptance criteria were met in all cases. The method could be successfully used for the analysis of Aprepitant in pharmaceutical dosage forms. Keywords: Aprepitant, Accuracy, Precision, Linearity, Mobile Phase and Validation

Two simple, sensitive selective accurate and economical spectrophotometric methods Method A&B for the determination of feropenem in bulk drug and pharmaceutical formulations (tablets) have been described in the present work. Method - A is based on the formation of  red colored ion-association complex between feropenem and methiline blue (MB) exhibiting absorption maximum at 650nm and obeying Beer’s law in the concentration range of 4-20µg/ml. The Method - B is based on the formation of Ion-association complex between feropenem and safranine-o(SFO) to yield an yellow colored chromogen exhibiting absorption maximum at 520nm(Method B) and obeying Beer’s law in the concentration range of 4-20µg/ml. Statistical analysis of the results has been carried out for the proposed methods revealing high accuracy and good precision. The proposed methods developed by the author could be successfully extended to the commercial pharmaceutical formulations (tablets) containing feropenem.

Two simple, sensitive selective accurate and economical spectrophotometric methods (Method-A&B) for the determination of Nateglinide in bulk drug and pharmaceutical formulations (tablets) have been described in the present work. Method - A is based on the formation of orange red colored ion-association complex between Nateglinide and Tropaeolineooo (TPooo) exhibiting absorption maximum at 470nm and obeying Beer’s law in the concentration range of 2.5-12.5µg/ml. The Method - B is based on the formation of Ion-association complex between Nateglinide and Alizarine Red S (ARS) to yield an yellow colored chromogen exhibiting absorption maximum at 440nm (Method B) and obeying Beer’s law in the concentration range of 2.5-12.5µg/ml. Statistical analysis of the results has been carried out for the proposed methods revealing high accuracy and good precision. The proposed methods developed by the author could be successfully extended to the commercial pharmaceutical formulations (tablets) containing Nateglinide.