Metformin

A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of alogliptin and metformin in tablet dosage form. The absorption maxima of the drugs were found to be 225 and 237 nm for alogliptin and metformin, respectively, in methanol, using a Shimadzu UV- visible spectrophotometer (model UV-1650). Alogliptin and metformin in obeyed beer’s law in the range of 0.05-0.25 µg/ml and 2-10 µg/ml, respectively. The correlation co-efficient value for metformin was found to be 0.99963 and 0.99936 at 225 nm and 237 nm, respectively. The correlation co-efficient value for alogliptin was found to be 0.99968 and 0.9333 at 225 nm and 237 nm, respectively. The method was validated for various parameters according to ICH guidelines. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method.

Gloriosa Superba is an Indian medicinal plant demonstrated to expect multiple pharmacology effects. In light of traditional claim of the plant in treatment of diabetes, the present study was carried out to evaluate the hypoglycemic actvity of the plant in STZ induced diabetics rats. The diabetic’s rats were given ethanolic extract of Gloriosa superb (EEGS) (50mg/kg, 100mg/kg, and 200mg/kg) and metformin (180mg/kg) for 28days.The effect of both treatments on body weight and blood glucose were assessed. Three doses of extract and metformin show significant hypoglycemic actvity in acute, sub-acute study. Body wt. of extract and metformin treated rats were maintained during the study period where as body wt. of untreated rats had gone down. The result obtained from the study scientifically proved the folkloric use of Gloriosa Superba as Hypoglycemic agent. Thus the plant can be key contributor in treatment of diabetes.