Methanol

A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of alogliptin and metformin in tablet dosage form. The absorption maxima of the drugs were found to be 225 and 237 nm for alogliptin and metformin, respectively, in methanol, using a Shimadzu UV- visible spectrophotometer (model UV-1650). Alogliptin and metformin in obeyed beer’s law in the range of 0.05-0.25 µg/ml and 2-10 µg/ml, respectively. The correlation co-efficient value for metformin was found to be 0.99963 and 0.99936 at 225 nm and 237 nm, respectively. The correlation co-efficient value for alogliptin was found to be 0.99968 and 0.9333 at 225 nm and 237 nm, respectively. The method was validated for various parameters according to ICH guidelines. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method.

U.V. spectrophotometric method for estimation of Eperisone hydrochloride as individual and in tablet formulation using graphical extrapolation method has been developed. Methanol was selected as solvent for estimation at 258 nm. Linearity was followed in the range of 2.5-17.5 mg/ml with correlation coefficient of 0.997. Detection limit and quantitation limit were found to be 0.082 mg/ml and 0.22 mg/ml .Developed method has followed all the criteria for validation as per ICH norms and found to be accurate, precise, reproducible and sensitive with negligible excipients interference. Accuracy of the developed was assessed using spiking technique on available tablet dosage forms. Results obtained greater than 98% meant good accuracy of method for analysis in any dosage form.