XRD.

Chitosan/Polyacrylamide containing nano-hydroxyapatite (HA) composite were prepared via in situ preparation technique. XRD (x-ray diffraction), (FTIR) Fourier transform infrared spectroscopy, (TEM) transmission electron microscopy, (SEM) scanning electron microscopy and (DSC) differential scanning colorimetery were used to characterize the prepared nano-composites before and after immersion in simulated body fluid (SBF).XRD confirm that addition of hydroxyapatite (HA) in the filling level has no effect on the amorphous nature of the base polymeric material with minor change in their crystallinity. FTIR spectra shows maintenance of the basic vibrational band corresponding to chitosan (CHI) and polyacrylamide (PAAm), while the change in the intensity of the peaks at 1092 and 1043 cm-1 indicates interaction between inorganic and organic matrix. Scanning electron micrographs of prepared samples before and after immersion in (SBF) shows a formation of crystalline regions of HA which confirmed by EDX measurements.