protein

ABSTRACTThree purified proteins G-I, G-II and G-III were obtained from Bacillus sp using the techniques of DEAE Sepharose Fast Flow Chromatography, filtration chromatography. anion exchange chromatography, molecular determination by SDS page gel electrophoresis (SDS-PAGE). The purity of G-I, G-II and G-III was measured by DEAE Sepharose Fast Flow Chromatography. G-I, G-II and G-III were measured by SDS-PAGE to have molecular weights of 14.4 k Da and 94.4 k Da, and three bands appear in the molecular weights of 46 k Da band 35 k Da and 32 k Da band respectively. The amino acid analysis of purified compound G-III was determined because greater antimicrobial activity of G-III compound. The amino acid analysis using technique of automatic amino acid analyzer (Shimatzu-High performance liquid chromatography LC 4A) 20 µl of the purified sample was injected into single column and analyzed using sodium buffer system. G-I, G-II and G-III inhibited the clinical pathogens Staphylococcus aureus and Pseudomonas aeruginosa. By antimicrobial activity of Agar well diffusion assay. Test the clinical pathogens shoe the greater activity of G-III and Zone of inhibition various concentration of (25 µl, 50 µl, 75 µl, 100 µl). G-III shows the maximum results of 18.2mm and 15.4mm against Staphylococcus aureus and Pseudomonas aeruginosa. Keywords: Bacillus sp; protein; purification; in vitro antimicrobial activity.

The present study has been made to study the effects of microcystin on the physiology of cockroach. Toxic effects of microcystin have been reported in rat, fish etc. this toxin effects the liver thus the metabolism of the organism is much affected. In cockroach, intracoelomic treatment of Microcystis extract showed some fluctuations in concentration of protein fractions especially in fractions 3, 4, 5 and 6. A common decrease in protein concentration is observed in 4, 5 and 6 but fraction 3 showed an increase in concentration. Fractions 7 and 8 failed to separate both in male and female cockroach. Reduced protein concentration for fraction 4 and 6 observed in male cockroach. The concentration of protein in the haemolymph generally decreases due to the microcystin treatment. Similar observation was recorded for the insects in spectrophotometric analysis of protein concentration.