purification

ABSTRACTGround water quality and its impact on human health in an industrial area near Tirupati, India has been assessed. Water samples were collected from 40 different locations of Vadamala Pet near Tirupati and analyzed for physicochemical parameters such as pH, electrical conductivity, total dissolved solids, total hardness, calcium, chlorides, sulphates, nitrates and dissolved oxygen etc. The found values of physicochemical parameters were compared with the World Health Organization (WHO) water quality standards. Based on the physico-chemical analysis and correlation studies, it was found that ground water of some of the studied areas was polluted and not suitable for drinking purpose. Thus the ground water of such areas needs purification before drinking. Keywords: Ground water, physicochemical parameters, purification, total dissolved solids, hardness.

ABSTRACTThree purified proteins G-I, G-II and G-III were obtained from Bacillus sp using the techniques of DEAE Sepharose Fast Flow Chromatography, filtration chromatography. anion exchange chromatography, molecular determination by SDS page gel electrophoresis (SDS-PAGE). The purity of G-I, G-II and G-III was measured by DEAE Sepharose Fast Flow Chromatography. G-I, G-II and G-III were measured by SDS-PAGE to have molecular weights of 14.4 k Da and 94.4 k Da, and three bands appear in the molecular weights of 46 k Da band 35 k Da and 32 k Da band respectively. The amino acid analysis of purified compound G-III was determined because greater antimicrobial activity of G-III compound. The amino acid analysis using technique of automatic amino acid analyzer (Shimatzu-High performance liquid chromatography LC 4A) 20 µl of the purified sample was injected into single column and analyzed using sodium buffer system. G-I, G-II and G-III inhibited the clinical pathogens Staphylococcus aureus and Pseudomonas aeruginosa. By antimicrobial activity of Agar well diffusion assay. Test the clinical pathogens shoe the greater activity of G-III and Zone of inhibition various concentration of (25 µl, 50 µl, 75 µl, 100 µl). G-III shows the maximum results of 18.2mm and 15.4mm against Staphylococcus aureus and Pseudomonas aeruginosa. Keywords: Bacillus sp; protein; purification; in vitro antimicrobial activity.