In-vitro drug release

Propranolol hydrochloride is a beta blocking agent which is used for the treatment of hypertension and it is highly lipophilic and completely absorbed after oral administration. The present study, Propranolol  hydrochloride  Mucoadhesive microspheres were formulated using  sodium alginate and the different concentration of polymers like Xanthan gum  and Guar gum by ionic gelation technique with  an  aim  to  controlled  release and improve bioavailability.  Six formulations were prepared by using different drug to polymer ratios, and evaluated for relevant parameters.  Depending upon the drug to polymer ratio, the percentage yield is found between 51.06±0.51% to 72.42±0.83 % in all formulations. The surface morphology of the microspheres was characterized by SEM. The prepared microspheres were discrete, spherical in shape and showed free flowing properties. The mean particle size of microspheres significantly increases with increasing polymer concentration and it was in the range between 23.10±0.12 µm to 33.66±0.41 µm. Among all the formulation, XG-III showed a high drug entrapment efficiency of 68.28±0.53 % and highest percentage swelling index of 67.75±0.06 %. The in-vitro drug release studies revealed that XG-III formulation is controlled and found to be 86.8±0.20 % and GG-III is found to be 89.8±0.11 % at the end of dissolution studies. The mechanism of drug release was evaluated using the linear regression coefficient. Stability studies of selected formulation showed good results. It could be also concluding that the all the formulations were shown satisfactory results and suitable for potential therapeutic uses.

Nasal Mucoadhesive gel of Glimepiride was prepared  for  Controlled release.  Carbopol 934p was used as a key ingredient which gives mucoadhesion property to the gel formulations. Different formulations were prepared by varying the concentrations of Carbopol 934p and Hydroxyl Propyl Methyl Cellulose K4M (HPMC K4M). These formulations were evaluated for parameters like pH, Drug content, Viscosity, Mucoadhesive strength, Gel strength, In-vitro drug release, In-vitro permeation and Drug excipient compatibility. In this formulation the release profile depend on the concentration of Carbopol 934p and HPMC K4M. A 32 factorial design was applied to see the effect of  variables Carbopol 934p (X1) and HPMC K4M (X2) on the response percentage drug release  as a dependent variable. In vitro release data were fitted to various models to ascertain kinetic of drug release. Regression analysis and analysis of variance were performed for dependent variables. The results of the F-statistics were used to select the most appropriate model.  Formulation containing Carbopol 934p(1.0%) and HPMC K4M(1.0%) was found to be  optimum. The studies indicate that the formulation was effective in providing In-vitro release of drug and the mucoadhesive formulation