Murraya koenigii

ABSTRACTThe objective of the present study was to assess the anti-inflammatory potential of combined extracts of Murraya koenigii and Mangifera indicausing the carrageenan induced rat paw edema method. The extraction yield in methanol was found to be 26.8 % for Murraya koenigii and 31.6 % for Mangifera indica. The findings of preliminary phytochemical analysis suggest the presence of alkaloids, saponin glycosides, phenolics, terpenoids, and flavonoids in the leaf of the Murraya koenigii while alkaloids and glycosides were not found to be present in Mangifera indica. The total phenolic content of the hydroalcoholic extracts of M. koenigii and M.indica were 34.27±1.7 and 43.11±2.1 GAE mg/g, respectively. The phenolic content was highest in the combined extract (MSE:MIE, 1:2) of all the three combinations with total phenolics 61.22±4.1GAE mg/g. The extracts were individually and in combination (1:1, 1:2 & 2:1) subjected to determination of anti-inflammatory potential by carrageenan induced rat paw edema method using ibuprofen as the standard drug. Ibuprofen at dose of 10 mg/Kg inhibited 69.23% edema after 4h of administration whereas the maximum edema inhibition exhibited by the combined extracts was 55.22% (MKE:MIE, 1:2)  at the end of 4h. Keywords: Murraya koenigii, Mangifera indica, carrageenan, Anti-inflammatory,paw edema, Extract

Murraya koenigii (Linn) is a plant of the Rutaceae family, well known in India as curry leaves. In the present study, evaluation of physicochemical characteristics; preliminary phytochemical parameters and pharmacological activities of methanol extract of dried leaves of Murraya koenigii Linn has been carried out. The preliminary phytochemical studies of the methanol extract of the leaves showed the presence of various phytochemical constituents such as alkaloids, tannins, flavonoids, steroids and saponins. . The aim of the present study was carried out with the objective of phytochemical screening and to evaluate the antiinflammatory, analgesic activity methanol extract of Murraya koenigii Linn Extract was studied for antiinflammatory activity by using carrageenan-induced hind paw edema in albino rats and the mean increase in paw volume and % inhibition in paw volume were measured plethysmometrically at different time intervals after carrageenan (1% w/v) injection. Extract was also evaluated for analgesic activity using Eddy's hot plate method and acetic acid-induced writhing method in albino rats. The methanol extract showed significant (P < 0.001) reduction in the carrageenan -induced paw edema and analgesic activity evidenced by increase in the reaction time by eddy's hot plate method and significantly reduced (P