Manikandan P

ABSTRACTThree purified proteins G-I, G-II and G-III were obtained from Bacillus sp using the techniques of DEAE Sepharose Fast Flow Chromatography, filtration chromatography. anion exchange chromatography, molecular determination by SDS page gel electrophoresis (SDS-PAGE). The purity of G-I, G-II and G-III was measured by DEAE Sepharose Fast Flow Chromatography. G-I, G-II and G-III were measured by SDS-PAGE to have molecular weights of 14.4 k Da and 94.4 k Da, and three bands appear in the molecular weights of 46 k Da band 35 k Da and 32 k Da band respectively. The amino acid analysis of purified compound G-III was determined because greater antimicrobial activity of G-III compound. The amino acid analysis using technique of automatic amino acid analyzer (Shimatzu-High performance liquid chromatography LC 4A) 20 µl of the purified sample was injected into single column and analyzed using sodium buffer system. G-I, G-II and G-III inhibited the clinical pathogens Staphylococcus aureus and Pseudomonas aeruginosa. By antimicrobial activity of Agar well diffusion assay. Test the clinical pathogens shoe the greater activity of G-III and Zone of inhibition various concentration of (25 µl, 50 µl, 75 µl, 100 µl). G-III shows the maximum results of 18.2mm and 15.4mm against Staphylococcus aureus and Pseudomonas aeruginosa. Keywords: Bacillus sp; protein; purification; in vitro antimicrobial activity.

Propranolol hydrochloride is a beta blocking agent which is used for the treatment of hypertension and it is highly lipophilic and completely absorbed after oral administration. The present study, Propranolol  hydrochloride  Mucoadhesive microspheres were formulated using  sodium alginate and the different concentration of polymers like Xanthan gum  and Guar gum by ionic gelation technique with  an  aim  to  controlled  release and improve bioavailability.  Six formulations were prepared by using different drug to polymer ratios, and evaluated for relevant parameters.  Depending upon the drug to polymer ratio, the percentage yield is found between 51.06±0.51% to 72.42±0.83 % in all formulations. The surface morphology of the microspheres was characterized by SEM. The prepared microspheres were discrete, spherical in shape and showed free flowing properties. The mean particle size of microspheres significantly increases with increasing polymer concentration and it was in the range between 23.10±0.12 µm to 33.66±0.41 µm. Among all the formulation, XG-III showed a high drug entrapment efficiency of 68.28±0.53 % and highest percentage swelling index of 67.75±0.06 %. The in-vitro drug release studies revealed that XG-III formulation is controlled and found to be 86.8±0.20 % and GG-III is found to be 89.8±0.11 % at the end of dissolution studies. The mechanism of drug release was evaluated using the linear regression coefficient. Stability studies of selected formulation showed good results. It could be also concluding that the all the formulations were shown satisfactory results and suitable for potential therapeutic uses.