American Journal Of Pharmacy And Health Research

ISSN NO.: 2321-3647
June 2018 Issue 6

Raoultella Ornithinolytica Urinary Tract Infections are on the Rise: A Case Report

Atif A. Katib1, Bassem A. Dakkak1, Husam A. Katib2, Sara K. Alem2

1. Department of Urology, King Abdul-Aziz hospital, Makkah, Saudi Arabia

2.Faculty of medicine, Ummal-Qura University, Makkah, Saudi Arabia


Community-acquire urinary tract infection (UTI) with Raoultella ornithinolytica is rare, with only 36 cases have been reported in the literature. The case in hand reports a 29 yrs. old lady with pelvic inflammatory disease who suffered from bothering irritative lower urinary tract symptoms (LUTS) ten days after removing an intrauterine contraceptive device. Urine culture recovered the rare bacterium of Raoultella ornithinolytica. Based on the sensitivity result, the patient has been treated with levofloxacin antibiotic and has responded favorably.

Keywords: Raoultella Ornithinolytica, Klebsiella, community-acquire UTI 


RP-HPLC Method Development and Validation for Simultaneous Estimation of Hydrocortisone and Acyclovir in Pharmaceutical dosage forms.

Ramavath Mohanbabu Naik1*, Syed Ahmed1, G. Naveen kumar Reddy2

1.Department  of Pharmaceutical Analysis and Quality Assurance, Gland institute of pharmaceutical science

2.Department  of Pharmacognosy, Surabhi Dayakar Rao college of Pharmacy


The Chromatographic condition were successfully developed for the separation of Acyclovir and     Hydrocortisone by using Agilent C8 column (25 cm x 4.6 mm i.d., 5 ?). A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Hydrocortisone and Acyclovir from pharmaceutical formulation. The method was carried out on a C8 (25 cm x 4.6 mm i.d., 5 ?) column with a mobile phase consisting of Methanol: water (adjusted to pH 3.0 using o-phosphoric acid) in the ratio of 80:20 v/v. The retention time of  Hydricortusone and Acyclovir was 3.50 min and 6.00 min respectively with the flow rate of 1mL/ min. Eluents were detected at 254 nm.  The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for Hydrocortisone and Acyclovir were R2=0.9995 and R2=0.9996 in the concentration range of 10-40µg. mL-1 , 20-80 µg. mL-1 respectively. The relative standard deviation for intra-day precision was lower than 2.0 %. The method was validated according to the ICH guidelines. The method was also found to be robust. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability.

Keywords: RP-HPLC, Hydrocortisone, Acyclovir, Agilent , Mobile Phase, Retention time.


In-Vitro Anticoagulant and Clot-Lysis Potential of Aqueous Extract of Datura Metel L.

Ashok A. Muchandi 1*, Nitin S. Thorat2, Suhas R. Mahanwar2

1.Department of Pharmacology, Adarsh College of Pharmacy, Vita, Maharashtra, India

2.Adarsh College of Pharmacy, Vita, Maharashtra, India


Thromboembolic disorders affecting millions of people worldwide. Presently available anticoagulants are closely related to antiplatelet drugs and thrombolytic drugs by manipulating the various pathways of blood coagulation but having limited use due serious side effects. A medicinal herb Datura metel belongs to family Solanaceae, has been widely used in traditional medicine. In the present study, different concentrations (20-100 µg/ml) of aqueous extract of leaves of Datura metel was evaluated for in-vitro anticoagulant and clot lysis activity. The results showed that, there was concentration dependent increase in anticoagulant activity as a measure of increased prothrombin time activity. Similarly, there was concentration dependent increase in percentage clot lysis activity. A maximum 23.81% clot lysis activity was reported at concentration 100 µg/ml of extract. Thus, it was concluded that, aqueous extract of leaves of Datura metel has potential in-vitro anticoagulant and clot lysis activity and may be beneficial in the treatment of cardiovascular diseases.

Keywords: Clot lysis, cardiovascular diseases, prothrombin time, anticoagulants, 


Formulation and Evaluation of Antimicrobial Activity of Topical Niosomal Gel of ErythromycinFormulation and Evaluation of Antimicrobial Activity of Topical Niosomal Gel of Erythromycin

Nettem Lalithajyotsna1*, Mohammad Sarfaraz Ali Ahmed2, Mahendar choudary3,Dr.vasudha Bakshi4

Department of Biotechnology, Anurag Group of Institutions (Formerly Lalitha College of Pharmacy) Venkatapur (v), Ranga Reddy-500088, Telangana, India.


Erythromycin is a macrolide antibiotic produced by Streptomyces erythreus. It inhibits bacterial protein synthesis by binding to bacterial 50S ribosomal subunits; binding inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins. Erythromycin may be bacteriostatic or bactericidal depending on the organism and drug concentration. The drugs in the form of topical gels avoid gastro-intestinal irritation, to overcome first pass effect and to maximize the drug concentration at the site of action. The main objective of the study is to formulate and evaluate the antimicrobial activity of erythromycin formulation for effective topical delivery of the drug. The antimicrobial activity of the erythromycin loaded gel is carried by cup-plate method and the zone of inhibition was noted. The current analysis demonstrates persistence release of drug, an increase in amount of drug maintenance into skin and enhanced permeation across the skin after entrapment of Erythromycin into niosomal topical gel.

Keywords: Niosomes, Erythromycin, Antimicrobial activity, topical delivery, cup-plate method, Niosomal Gel and Skin Retention.