American Journal Of Pharmacy And Health Research

ISSN NO.: 2321-3647
November 2022 Issue 11

Metformin affects alternative splicing of the estrogen receptor alpha gene in breast cancer cells

Yoshihiro Harada1, Tomoko Tanaka2, Yuriko Hamaguchi2,Yuta Horita1, Masayoshi Mori1, Yusuke Murata1, Munechika Enjoji1, Kenji Ohe1,*

1. Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.

2.Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan


Metformin ameliorates insulin resistance and reported to have prophylactic potential in breast cancer. The estrogen receptor-alpha gene is known to be alternatively spliced to the ER?46 isoform with decreased expression in tamoxifen-resistant breast cancer cells. We show here that metformin had a differential effect on its alternative splicing in MCF-7 and its tamoxifen-resistant derivative, TAMR1 cells. Metformin altered the tamoxifen-induced expression of ER?46 and treatment of cell-transplanted nude mice showed decreased tumor weight only in TAMR1 cell tumors. This is the first report to show metformin specifically involved in alternative splicing of genes in breast cancer cells.

Keywords: Estrogen receptor-alpha (ER?), estrogen, tamoxifen, metformin


Leiomyomatous polyp presenting as Intussusception in an elderly female - A Rare Case Report

Bushra Siddiqui1,Gouthami N1 , Saqib Ahmed*1 , Zeeshan Nahid1,Shahbaz Habib Faridi2

1. Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Uttar Pradesh, India

2. Department of General Surgery, Jawaharlal Nehru Medical College, Aligarh Muslim University, Uttar Pradesh, India


Intussusception is quite common in the gastrointestinal tract and that too in the paediatric population though the aetiology is mostly idiopathic (90%). While in adults it is rare and is usually caused by an identifiable underlying lesion mainly neoplasm (70-90%) which usually acts as a lead point. It has a non-specific clinical presentation with a limited role of history and examination in the final diagnosis and Computed tomography is considered the gold standard investigation in establishing the diagnosis. We present an unusual case of jejuno-jejunal intussusception which was caused by a leiomyoma polyp. Thus an Exploratory laparotomy was done, resecting the polyp with the jejunal segment and the diagnosis was confirmed by histopathological examination.

Keywords: Intussusception, Jejunal, Leiomyoma, Histopathology