Metformin affects alternative splicing of the estrogen receptor alpha gene in breast cancer cells

Yoshihiro Harada¹, Tomoko Tanaka², Yuriko Hamaguchi²,Yuta Horita¹, Masayoshi Mori¹, Yusuke Murata¹, Munechika Enjoji¹, Kenji Ohe^1,*

1. Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.

2.Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan

Abstract Certificate Download PDF (294) Journal DOI : 10.5281/zenodo.7412528 Google Citation

ABSTRACT

Metformin ameliorates insulin resistance and reported to have prophylactic potential in breast cancer. The estrogen receptor-alpha gene is known to be alternatively spliced to the ER?46 isoform with decreased expression in tamoxifen-resistant breast cancer cells. We show here that metformin had a differential effect on its alternative splicing in MCF-7 and its tamoxifen-resistant derivative, TAMR1 cells. Metformin altered the tamoxifen-induced expression of ER?46 and treatment of cell-transplanted nude mice showed decreased tumor weight only in TAMR1 cell tumors. This is the first report to show metformin specifically involved in alternative splicing of genes in breast cancer cells.

Keywords: Estrogen receptor-alpha (ER?), estrogen, tamoxifen, metformin

Leiomyomatous polyp presenting as Intussusception in an elderly female - A Rare Case Report

Bushra Siddiqui¹,Gouthami N¹ , Saqib Ahmed*¹ , Zeeshan Nahid¹,Shahbaz Habib Faridi²

1. Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Uttar Pradesh, India

2. Department of General Surgery, Jawaharlal Nehru Medical College, Aligarh Muslim University, Uttar Pradesh, India

Abstract Certificate Download PDF (174) Journal DOI : 10.5281/zenodo.7412541 Google Citation

ABSTRACT

Intussusception is quite common in the gastrointestinal tract and that too in the paediatric population though the aetiology is mostly idiopathic (90%). While in adults it is rare and is usually caused by an identifiable underlying lesion mainly neoplasm (70-90%) which usually acts as a lead point. It has a non-specific clinical presentation with a limited role of history and examination in the final diagnosis and Computed tomography is considered the gold standard investigation in establishing the diagnosis. We present an unusual case of jejuno-jejunal intussusception which was caused by a leiomyoma polyp. Thus an Exploratory laparotomy was done, resecting the polyp with the jejunal segment and the diagnosis was confirmed by histopathological examination.

Keywords: Intussusception, Jejunal, Leiomyoma, Histopathology