Photobiomodulation efficacy of 808 nm Low Level Laser therapy on Lipopolysaccharide compromised Immune status: An Experimental study in Rats
Richa Rathor*,
Sukanya Srivastava, Asheesh Gupta, Geetha Suryakumar
Defence Institute of Physiology and Allied
Sciences, Lucknow Road, Timarpur
ABSTRACT
The current study aimed to assess the efficacy of two unlike laser
wavelengths (655 nm and 808 nm) used as photobiomodulation or low-level laser
therapy (LLLT) on the immune status in male rats. Sixty-five male Sprague–Dawley
rats were considered in the experiments which were further divided into three
groups. For all the three groups, LPS (lipopolysaccharide) was used as an
immunostimulant and it was administered 5mg/kg via intraperitonially (ip) route
for 3 consecutive days. In the first group, 655 nm LLLT (power 150 mW) was
investigated against LPS induced immune response. Second group investigated 808
nm LLLT (power 150 mW) against LPS induced immune function. After finding out
the better responsive LLLT, third group investigated 808 nm LLLT with different
power ie 75 mW and 150 mW. The results indicated that 655 nm LLLT at 150 mW
power was not restored LPS associated TNF-? and IL-1? content. While, LPS
induced enhance IL-1? and TNF-? level was significantly decreased in 808 nm
LLLT group. Further, comparison was studied in 808 nm LLLT at different power
ie 150 mW and 75 mW and pro-inflammatory cytokines estimation was quantified.
The outcome of the study concluded that 808 nm LLLT has anti-inflammatory
activity at 150 mW power. Hence,
the further research work is required in this direction to advise that 808 nm LLLT at 150 mW could be considered as
a treatment to protect inflammatory diseases.
Keywords: Photobiomodulation; low-level laser therapy
(LLLT); immune status; 655nm; 808nm; in vivo