Evaluation of the Liver Toxicity and Nephrotoxicity of Creatinor, a Natural Recipe Used in the Treatment of Renal Failure in Côte d'Ivoire
1.Laboratory of Biology and Health,
Specialty Animal Physiology, Phytotherapy and Pharmacology, Biosciences
Training and Research Unit (UFR), Félix Houphouët-Boigny University, Abidjan,
Côte d'Ivoire.
2.Laboratory of Animal Biological
Sciences, Specialty Animal Physiology, Phytotherapy and Pharmacology, Unit of
Training and Research (UFR) Science and Technology, Alassane Ouattara
University, Bouaké, Côte d'Ivoire.
3.Laboratory of Biodiversity and
Tropical Ecology, Specialty Animal Physiology and Pharmacology, University Jean
Lorougnon Guede, Daloa, Côte d'Ivoire.
4. Department of
Medical and Fundamental Biochemistry, Institute Pasteur in Côte d'Ivoire.
ABSTRACT
Creatinor is a natural
recipe used in Côte d'Ivoire by traditional therapists to treat kidney failure.
However, its toxicity has not yet been scientifically studied. The aim of this
study is to assess the safety threshold of Creatinor use in mice and the toxic
potential of this substance on the kidneys and liver of healthy rats. The acute
oral toxicity study of Creatinor, in accordance with OECD 423 recommendations,
shows that this substance is non-toxic in mice. The sub-acute toxicity study of
Creatinor in healthy rats, in accordance with OECD guideline 407, showed that
doses of 100 and 200 mg/kg bw caused, in a dose-dependent manner, decreases in
the relative weights of the kidneys and liver of treated rats and, from the
21st day of treatment, increases in the levels of creatinine, urea, ALAT and
ASAT. Creatinor also induced disorganisation of the histological structures of
the kidneys and liver, with tissue necrosis. Creatinor, administered to healthy
rats over a long period at doses of 100 and 200 mg/kg bw, is said to have
nephrotoxic and hepatotoxic effects. At 200 mg/kg bw, these effects were more marked
and more precocious, with adverse effects on body development. On the other
hand, Creatinor at 50 mg/kg bw has no effect.
Key words:
Creatinor, toxicity, nephrotoxicity, hepathotoxicity, renal failure